Vialinin A and thelephantin G, potent inhibitors of tumor necrosis factor-α production, inhibit sentrin/SUMO-specific protease 1 enzymatic activity

Yasukiyo Yoshioka; Daisuke Namiki; Mao Makiuchi; Kouichi Sugaya; Jun-Ichi Onose; Hitoshi Ashida; Naoki Abe

doi:10.1016/j.bmcl.2016.07.051

Vialinin A and thelephantin G, potent inhibitors of tumor necrosis factor-α production, inhibit sentrin/SUMO-specific protease 1 enzymatic activity

Bioorg Med Chem Lett. 2016 Sep 1;26(17):4237-40. doi: 10.1016/j.bmcl.2016.07.051. Epub 2016 Jul 27.

Authors

Yasukiyo Yoshioka¹, Daisuke Namiki², Mao Makiuchi², Kouichi Sugaya², Jun-Ichi Onose², Hitoshi Ashida³, Naoki Abe⁴

Affiliations

¹ Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 156-8502, Japan; Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo 657-8501, Japan.
² Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 156-8502, Japan.
³ Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo 657-8501, Japan; Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 651-8501, Japan.
⁴ Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: abe@nodai.ac.jp.

PMID: 27491710
DOI: 10.1016/j.bmcl.2016.07.051

Abstract

Several p-terphenyl compounds have been isolated from the edible Chinese mushroom Thelephora vialis. Vialinin A, a p-terphenyl compound, strongly inhibits tumor necrosis factor-α production and release. Vialinin A inhibits the enzymatic activity of ubiquitin-specific peptidase 5, one of the target molecules in RBL-2H3 cells. Here we examined the inhibitory effect of p-terphenyl compounds, including vialinin A, against sentrin/SUMO-specific protease 1 (SENP1) enzymatic activity. The half maximal inhibitory concentration values of vialinin A and thelephantin G against full-length SENP1 were 1.64±0.23μM and 2.48±0.02μM, respectively. These findings suggest that p-terphenyl compounds are potent SENP1 inhibitors.

Keywords: SENP1; SUMO; TNF-α; Vialinin A.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales / chemistry
Agaricales / metabolism
Animals
Cell Line
Humans
Kinetics
Protein Binding
Rats
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / isolation & purification
SUMO-1 Protein / antagonists & inhibitors
SUMO-1 Protein / metabolism*
Terphenyl Compounds / chemistry
Terphenyl Compounds / metabolism*
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Tumor Necrosis Factor-alpha / metabolism*

Substances

Recombinant Proteins
SUMO-1 Protein
Terphenyl Compounds
Tumor Necrosis Factor-alpha
thelephantin G
vialinin A